Characterisation of Phytochemical Content of Seagrass Ethanol Extract Using Gas Chromatography Mass Specrometry (GC-MS)

Sri Wahyuni, Uswatun Hasanah, Ismail Sulaiman, Munandar Munandar, Muhammad Rizal

Abstract


Research on the bioactive potential of marine biological resources is increasingly growing, especially in marine plants such as seagrasses that produce secondary metabolites. This study aims to characterize the phytochemical content of the ethanol extract of Thalassia hemprichii seagrass from Lhok Timon beach, Aceh Jaya, using the Gas Chromatography-Mass Spectrometry (GC-MS) method. The main issue addressed is the lack of quantitative data on the phytochemical composition of this seagrass, particularly secondary metabolites. Extraction was conducted using the maceration method with 96% ethanol, followed by GC-MS analysis to identify bioactive compounds. The analysis results revealed 38 compounds, with D-Fructose, diethyl mercaptal, pentaacetate as the dominant one, displaying potential biological activities such as antimicrobial, antioxidant, anti-inflammatory, and antifungal properties. Other significant compounds include fatty acids and phenols that support antioxidant activity. The discussion emphasizes the potential development of these compounds for pharmaceutical and industrial applications, given their bioactive properties. In conclusion, T. hemprichii seagrass contains promising phytochemicals for further development as bioactive agents in the health sector


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Al-Rubaye, A. F., Hameed, I. H., & Kadhim, M. J. (2017). A review: uses of Gas Chromatography-Mass Spectrometry (GC-MS) technique for analysis of bioactive natural compounds of some plants. International Journal of Toxicological and Pharmacological Research, 9(01), 81-85.

D., Carmo, M. A. V. do, Azevedo, L., Archilha, M. V. L. R., & Scharf, D. R. (2021). Selina-1,3,7(11)-trien-8-one and Oxidoselina-1,3,7(11)-trien-8-one from Eugenia uniflora leaf essential oil and their cytotoxic effects on human cell lines. Molecules, 26(740), 1–11.

Baransano, H. K., & Mangimbulude, J. C. (2011). Eksploitasi dan konservasi sumberdaya hayati laut dan pesisir di Indonesia. Jurnal Biologi Papua, 3(1), 39–45.

Chinnadurai, V., Viswanathan, P., Kalimuthu, K., Vanitha, A., Ranjitha, V., & Pugazhendhi, A. (2019). Comparative studies of phytochemical analysis and pharmacological activities of wild and micropropagated plant ethanol extracts of Manihot esculenta. Biocatalysis and Agricultural Biotechnology, 19, 101-166.

Dhuha, S., Bodhi, W., & Kojong, N. (2016). Aktivitas antibakteri ekstrak etanol daun lamun (Syiringodium isoetifolium) terhadap bakteri Pseudomonas aeruginosa. Pharmacon Jurnal Ilmiah Farmasi 5(1), 231–237.

Dinata, H. N., Henri, H., & Adi, W. (2022). Analisis habitat Gastropoda pada ekosistem lamun di Perairan Pulau Semujur, Bangka Belitung. Jurnal Ilmiah Sains, 22(1), 49-59.

Febrian Illahi, G., Karina, S., & Irwan. (2021). Studi literatur potensi lamun di beberapa Perairan Aceh. Jurnal Kelautan dan Perikanan Indonesia, 1(2), 94–103.

Hasan, H., Suryadi, A. M. A., & Djufri, Z. (2022). Uji aktivitas antidiabetes ekstrak etil asetat daun lamun (Enhalus acoroides) Pada Mencit (Mus musculus). Journal Syifa Sciences and Clinical Research (JSSCR), 4(1), 293–305.

Hayano, K., & Shirahama, H. (1996). Diethyl Ether–Boron Trifluoride (1/1) induced transannular cyclization reaction of Humulene 2,3-Epoxide. Bulletin of the Chemical Society of Japan, 69(2), 459–464.

Höfs, R., Walker, M., & Zeeck, A. (2000). Hexacyclinic acid, a polyketide from Streptomyces with a novel carbon skeleton. Angewandte Chemie - International Edition, 39(18), 3258–3261.

Jameel M. Al-Khayri, 2, G. R. S., Nagella, P., Joseph, B. V., Alessa, F. M., & Al-Mssallem, M. Q. (2022). Flavonoids as potential anti-inflammatory molecules: a review. Molecules 2022, 27, 2901.

Jiang, Z., Mou, J., Feng, J., Zhang, S., Li, D., & Liu, Y. (2024). Comparative analysis of volatile components in chi-nan and ordinary agarwood aromatherapies: implications for sleep improvement. Pharmaceuticals, 17(9), 1196.

K. Momin, & Thomas, S. C. (2020). GC–MS Analysis of antioxidant compounds present in different extracts of an endemic plant Dillenia scabrella (Dilleniaceae). Leaves and Barks. 11(5), 2262–2273.

Kakkar, S., & Narasimhan, B. (2019). A comprehensive review on biological activities of oxazole derivatives. BMC Chemistry, 13(16), 1-24.

Kannan, R. R. R., Arumugam, R., & Anantharaman, P. (2010). Antibacterial potential of three seagrasses against human pathogens. Asian Pacific Journal of Tropical Medicine, 3(11), 890–893.

Kazimírová, V., & Rebroš, M. (2021). Production of aldehydes by biocatalysis. International Journal of Molecular Sciences, 22(9), 4949.

Kumaradevan, G., Damodaran, R., Mani, P., Dineshkumar, G., & Jayaseelan, T. (2015). Phytochemical screening and GC-MS analysis of bioactive components of ethanol leaves extract of Clerodendrum phlomidis (L.). American Journal of Biological and Pharmaceutical Research, 2(3), 142–148.

Kurniasari, S., Djailani, F. M., Amir, A., Irsan, Yunus, M., & Fuadi, N. (2023). Potensi lamun sebagai antikanker : review artikel. Jurnal Sains Fisika, 3(1), 9–18.

Kusuma, A. H., Nugraha, A. H., & Shasika, A. B. (2023). Antibacterial activity of seagrass extract against pathogen bacteria of Escherichia coli strain Multi Drug Resistance (MDR). Jurnal Biologi Tropis, 23(4), 171–178.

Maarisit, I., Angkouw, E. D., Mangindaan, R. E. P., Rumampuk, N. D. C., Manoppo, H., & Ginting, E. L. (2021). Isolation and antibacterial activity test of seagrass epiphytic symbiont bacteria Thalassia hemprichii from Bahowo Waters, North Sulawesi. Jurnal Ilmiah Platax, 9(1), 115–122.

Maulani, M., Nurjanah, S., & Lembong, E. (2022). Uji aktivitas antijamur pada α-guaiene minyak nilam terhadap jamur Microsporum gypseum ATCC 14683 dan Tricophyton mentagrophytes ATCC 16404. Jurnal Keteknikan Pertanian Tropis Dan Biosistem, 10(1), 74–83.

Maulida, A., Afdhal El Rahimi, S., & Kurnianda, V. (2018). Structure of the seagrass community with variation of depth in Ahmad Rhang Manyang Bay, Aceh Besar District. Jurnal Ilmiah Mahasiswa Kelautan dan Perikanan Unsyiah, 3(2), 1–11.

Melati, P. (2021). Uji aktivitas antioksidan, sitotoksisitas dan GC-MS ekstrak metanol alga hijau Boergesenia forbesii (Harvey) Feldmann dari Pantai Panjang Bengkulu. Jurnal Pengelolaan Laboratorium Sains dan Teknologi, 1(1), 10–24.

Meng, S., Chen, J., Xu, P., Qu, J., Fan, L., Chao, S., & Qiu, L. (2014). Hepatic antioxidant enzymes SOD and CAT of Nile Tilapia (Oreochromis niloticus) in response to pesticide methomyl and recovery pattern. Bull Environ Contam Toxicol 92, 388–392.

Mohammed, G. J., Hameed, I. H., & Kamal, S. A. (2018). Determination of bioactive chemical compounds of Aspergillus flavus using GC/MS and ftir and evaluation of its anti-microbial activity. Indian Journal of Public Health Research and Development, 9(3), 247–253.

Nurafni, & Nur, M. R. (2018). Aktivitas antifouling senyawa bioaktif dari lamun di Perairan Pulau Morotai. Jurnal Ilmu Kelautan Kepulauan 1(2), 107–112.

Owolabi, O. O., James, D. B., Sani, I., Andongma, B. T., Fasanya, O. O., & Kure, B. (2018). Phytochemical analysis, antioxidant and anti-inflammatory potential of FERETIA apodanthera root bark extracts. BMC Complementary and Alternative Medicine, 18(1), 1–9.

Paputungan, Z., Wonggo, D., & Kaseger, B. E. (2017). Uji fitokimia dan aktivitas antioksidan buah mangrove Sonneratia alba di Desa Nunuk, Kecamatan Pinolosian, Kabupaten Bolaang Mongondow, Selatan Sulawesi Utara. Jurnal Media Teknologi Hasil Perikanan, 5(3), 96-102.

Paryono, M., Dewi, E. N., & Fahmi, A. S. (2021). Aktivitas antioksidan ekstrak lamun Enhalus acoroides yang dikeringkan dengan metode pengeringan berbeda. Jurnal Ilmu Dan Teknologi Perikanan, 3(1), 10–15.

Pradhan, S., & Dubey, R. C. (2021). GC–MS analysis and molecular docking of bioactive compounds of Camellia sinensis and Camellia assamica. Archives of Microbiology, 203(5), 2501–2510.

Ramalakshmi, P., Sama, A. K. V., & Annapoorani, P. (2023). Identification of bioactive components and its biological activities of Toddalia asiatica root extract– a GC-MS study. Biochemical and Cellular Archives, 23(1), 555–560.

Rasyid, A., Putra, M. Y., & Yasman. (2023). Antibacterial and antioxidant activity of sea cucumber extracts collected from Lampung waters, Indonesia. Kuwait Journal of Science, 50(4), 615–621.

Singh, B. K., & Maurya, A. (2023). Antioxidant Activity of Essential Oils: A Mechanistic Approach. Plant Essential Oils: From Traditional to Modern-Day Application, 59–76.

Subhashini, P., Dilipan, E., Thangaradjou, T., & Papenbrock, J. (2013). Bioactive natural products from marine angiosperms: abundance and functions. Natural Products and Bioprospecting, 3(4), 129–136.

Suleman, I. F., Sulistijowati, R., Manteu, S. H., & Nento, W. R. (2022). Identifikasi Senyawa Saponin Dan Antioksidan Ekstrak Daun Lamun (Thalassia hemprichii). Jambura Fish Processing Journal, 4(2), 94–102.

Surahmaida, Umarudin, Rani, A. W., & Dewi, N. C. (2021). Skrining Fitokimia Senyawa Metabolit Sekunder Ekstrak Metanol Daun Jarak Pagar (Jatropha curcas) dengan GCMS. Journal of Pharmacy and Science, 6(1), 25–30.

Swamy, M. K., & Sinniah, U. R. (2015). A comprehensive review on the phytochemical constituents and pharmacological activities of Pogostemon cablin Benth.: An aromatic medicinal plant of industrial importance. Molecules, 20(5), 8521–8547.

Umamaheshwari, R., Thirumaran, G., & Anantharaman, P. (2009). Potential antibacterial activities of seagrasses from vellar estuary Southeast Coast of India. Advances in Biological Research, 3, 140–143.

Varshini, R., Manokiruthika, V., Hariprasath, L., Muthusami, S., & Suganyadevi, P. (1953). Cycloheptasiloxane, tetradecamethyl identified from petroleum ether fraction of Sargassum wightii by Gas Chromatography-Mass Spectrometry Analysis.

Vennila, S., Radha, P., Tilak, M., & Kumaran, K. (2023). GC-MS analysis of bioactive compounds on hexane leaf extract of Anogeissus latifolia (Roxb. Ex Dc.) Wall . Ex Bedd ). The Pharma Innovation Journal, 12(5), 4665–4668.

Widyasanti, A., Nurjanah, S., Nurhadi, B., & Osman, C. P. (2023). Optimization of the vacuum fractional distillation process for enhancing the α-guaiene of patchouli oil with Response Surface Methodology. Separations, 10, 469.

Wu, Y. X., Lu, W. W., Geng, Y. C., Yu, C. H., Sun, H. J., Kim, Y. J., Zhang, G., & Kim, T. (2020). Antioxidant, antimicrobial and anti-inflammatory activities of essential oil derived from the wild rhizome of Atractylodes macrocephala. Chemistry and Biodiversity, 10.1002/cbdv.202000268.

Yang, W.-H., Liu, Y.-H., Liang, J.-L., Lin, Z.-X., Kong, Q.-L., Xian, Y.-F., Guo, D.-Q., Lai, Z.-Q., Su, Z.-R., & Huang, X.-Q. (2017). β-Patchoulene, isolated from patchouli oil, suppresses inflammatory mediators in LPS-stimulated RAW264.7 macrophages. European Journal of Inflammation, 15(2), 136–141.

Zhu, P., Zheng, J., Yan, J., Li, Z., Li, X., & Geng, H. (2023). Design, synthesis, and biological evaluation of N′-Phenylhydrazides as potential antifungal agents. International Journal of Molecular Sciences, 24(20), 15120.




DOI: https://doi.org/10.35308/jpterpadu.v5i2.12394

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