Effect of Germination on Antioxidant Activity of Peanut Sprouts (Arachis Hypogaea)

Afwa Hayuningtyas, Suci Eka Putri, Rinawati Rinawati, Khairunnas Khairunnas, Marniati Marniati

Abstract


Peanuts (Arachis hypogaea L.) are plant-based foods that are abundantly available in Indonesia which also have high economic value and are an essential source of protein for many people. In Aceh, peanuts are one of the leading commodities, especially in the districts of Pidie, West Aceh, Aceh Jaya, Nagan Raya, Southwest Aceh and South Aceh. Apart from being rich in protein, it also contains fibre, antioxidants, and other good health components. During germination, proteins, starches, and lipids are metabolised by enzymes. These components are used as building blocks for growth and as a source of energy for respiration. Germination has the advantage of increasing several levels of nutrients, eliminating oligosaccharides that cause flatulence, and increasing several types of vitamins. Previous research reported that soybean germination significantly increased isoflavone levels and antioxidant activity of fresh tempeh flour produced. In this study, the antioxidant activity of peanut sprouts was identified using the DPPH (2,2-diphenyl-1-picryl-hydrazine-hydrate) radical scavenging method in three different germination times. The result shows that the most optimal percentage of antioxidant activity is the 48-hour treatment (88.24%). It also shows that the germination treatment can increase the per cent of antioxidant activity compared to peanuts without germination. 


Keywords


Antioxidant activity; Germination; Peanut sprout.

Full Text:

PDF

References


Aguilera, Y.; Herrera, T.; Benítez, V.; Arribas, S.M.; López de Pablo, A.L.; Esteban, R.M.; Martín-Cabrejas, M.A. (2015). Estimation of the scavenging capacity of melatonin and other antioxidants: Contribution and evaluation in germinated seeds. Food Chem. 170, 203–211.

Aguilera, Y.; Liébana, R.; Herrera, T.; Rebollo-Hernanz, M.; Sanchez-Puelles, C.; Benítez, V.; Martín-Cabrejas, M.A. (2014). Effect of illumination on the content of melatonin, phenolic compounds, and antioxidant activity during germination of lentils (Lens culinaris L.) and kidney beans (Phaseolus vulgaris L.). J. Agric Food Chem. 62, 10736–10743.

Amrun, H. M, Umiyah, dan E. U. Umayah. (2007). Uji Aktivitas Antioksidan Ekstrak Air dan Ekstrak Metanol Beberapa Varian Buah Kenitu (Chrysopylum cainito L.) dari Daerah Jember . Berkala Penelitian Hayati 13. Jurusan Biologi Universitas Jember.

Arnao, M.B.; Hernández-Ruiz, J. (2015). Functions of melatonin in plants: A review. J. Pineal. Res. 59, 133–150.

Astawan, M. (2020). Pengaruh Germinasi Kedelai terhadap Komposisi Proksimat dan Komponen Bioaktif IsoflavonTempe Segar dan Semangit. JURNAL PANGAN, 29(1), 35-44.

Bau, H.-M.; Villaume, C.; Nicolas, J.-P.; Méjean, L. (1997). Effect of germination on chemical composition, biochemical constituents and anti-nutritional factors of soya bean (Glycine max) seeds. J. Sci. Food Agric. 73, 1–9.

BPS. (2014). Statistik Keuangan Pemerintah Kabupaten/Kota 2014- 2015. Jakarta: Badan Pusat Statistik.

Brand Willians, W., Cuvelier, M. E., Berset. C. J. L. F. S & Technology. (1995). Use a free radical method to evaluate antioxidant activity. 28, 25–30.

Chang, S.S., Bostric-Matijasevic, O.A.L.Hsieh, dan C.L.Huang. (1977). Natural Antioxidants from Rosemary and Sage. J. Food.Sci. 42:574.

Chavan, J. and S. S. Kadam. (1989). Nutritional improvement of cereals by sprouting. Food Sci. and Nutri. 28: 401-437.

Dueñas, M. (2015). Effect of germination and elicitation on phenolic composition and bioactivity of kidney beans. Food Res. Int. 70,55–63.

Dungir, S. G., Katja, D. G., & Kamu, V. S. (2012). Aktivitas antioksidan ekstrak fenolik dari kulit buah manggis (Garcinia mangostana L.). Jurnal Mipa, 1(1), 11-15.

Ferdiawan, N., Nurwantoro, N., & Dwiloka, B. (2019). Pengaruh Lama Waktu Germinasi terhadap Sifat Fisik dan Sifat Kimia Tepung Kacang Tolo (Vigna unguiculata L). Jurnal Teknologi Pangan, 3(2), 349-354.

Ganesan, K.; Xu, B. (2018). A critical review on phytochemical profile and health-promoting effects of mung bean (Vigna radiata). Food Sci. Hum. Wellness. 7, 11–33.

Guo, X.; Li, T.; Tang, K.; Liu, R.H. (2012). Effect of germination on phytochemical profiles and antioxidant activity of mung bean sprouts (Vigna radiata). J. Agric Food Chem. 60, 11050–11055.

Istiani, Y. (2010). Karakterisasi senyawa bioaktif isoflavon dan uji aktivitas antioksidan dari ekstrak etanol tempe berbahan baku koro pedang (Canavalia ensiformis) (Doctoral dissertation, UNS (Sebelas Maret University).

Kusumaputri, V. S. (2010). Karakteristik Pertumbuhan dan Produksi Delapan Varietas Kacang Tanah (Arachis hypogea L). Skripsi. Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor.

Losada-Barreiro, S., Sezgin-Bayindir, Z., and, F. P.-M., & Bravo-Díaz, C. (2022). Biochemistry of Antioxidants: Mechanisms and Pharmaceutical Applications. Biomedicines, 10(12). doi: 10.3390/biomedicines10123051.

Mooy, H., Nuraini, A., & Sumadi, S. (2021). Respons perkecambahan benih jagung manis terhadap konsentrasi dan lama perendaman giberelin pada suhu lingkungan yang berbeda. Kultivasi, 20(1), 53-61.

Mubarak, A.E. (2005) Nutritional composition and antinutritional factors of mung bean seeds (Phaseolus aureus) as affected by some home traditional processes. Food Chem. 89, 489–495.

Pal M, Misra K, Dhillon G, Verma M. (2014). Antioxidants. Chapter: Biotransformation of Waste Biomass into High Value Biochemicals. DOI: 10.1007/978-1-4614-8005-1_6.

Polak, R., Philips, E. M., & Campbell, A. (2015). Legumes: health benefit and culinary approaches to increase intake. Clinical Diabetes, 33 (4). 198–205.

Saleh, H.M.; Hassan, A.A.; Mansour, E.H.; Fahmy, H.A.; El-Bedawey, A.E.-F.A. (2019) Melatonin, phenolics content and antioxidant activity of germinated selected legumes and their fractions. J. Saudi. Soc. Agric Sci. 18, 294–301.

Shah, A. R., Ara, N., & Shafi, G. (2011). Seed priming with phosphorus increased germination and yield of okra. African Journal of Agricultural Research, 6(16), 3859-3876.

Shofi, M. (2017). Pengaruh logam berat merkuri clorida (HGCL2) terdadap perkecambahan biji kacang hijau (Vigna radiata L.). Jurnal Wiyata Penelitian Sains dan Kesehatan. 4(1): 84-89.

Sutopo, Lita. (2002). Teknologi Benih. Rajawali. Jakarta.

Uchegbu, N. N. dan C. N. Ishiwu. (2016). Germinated Pigeon Pea (Cajanus cajan); A Novel Diet for Lowering Oxidative Stress and Hyperglycemia. Food Science and Nutrition. 4(5): 772-777.

Van Hung, P.; Hoang Yen, N.T.; Lan Phi, N.T.; Ha Tien, N.P.; Thu Trung, N.T. (2020) Nutritional composition, enzyme activities and bioactive compounds of mung bean (Vigna radiata L.) germinated under dark and light conditions. LWT.133, 110100.

Vidal-Valverde, C., Frias, J., Sierra, I., Blázquez, I., Lambein, F., & Kuo, Y.-H. (2002). New functional foods by germination: Effect on the nutritive value of beans, lentils and peas. Researchgate.

Walters, R. G., Rogers, J. J., Shephard, F., and Horton, P. (2000). Acclimation of Arabidopsis thaliana to the light environment: the role of photoreceptors. Planta, 209(4):517-527.




DOI: https://doi.org/10.35308/j-kesmas.v10i1.7198

Refbacks

  • There are currently no refbacks.


Managed by Fakultas Kesehatan Masyarakat

Published by Universitas Teuku Umar
Website: http://jurnal.utu.ac.id/jkesmas
Email: jkemas@utu.ac.id

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.